Sodium nitroprusside enhances biomass and gymnemic acids production in cell suspension of Gymnema sylvestre (Retz.) R.Br. ex. Sm.

Gymnemic acids (a group of triterpenoid saponins) found in Gymnema sylvestre (Retz.) R.Br. ex Sm. works as the main hypoglycaemic active components. These can be potential pharmaceutical ingredient (API) to used by industries modern medicines against diabetes. The present study aims investigate effectiveness sodium nitroprusside (SNP) treatment for enhancement cell suspension culture biomass and quantify production deacylgymnemic acid, gymnemagenin, gymnemic acid IV XVII contents. Callus was obtained from vitro derived leaves G. on MS medium fortified with 3.0 mg/L 2, 4-d (2, 4-dichlorophenoxyacetic acid) 1.0 Kn (Kinetin), same were further produce cultures. Cell suspensions exposed different concentrations SNP (5, 10, 20 40 ?M) data collected at 20, 30 days. Out tested concentrations, ?M had highest level growth (398.94 ± 8.32 g/L Fresh weight (FCW) 40.00 0.75 Dry (DCW) 40-day compared control (MS + Kn). High-performance liquid chromatography analysis showed that maximum accumulation (5.51 mg/g DCW), gymnemagenin (2.80 DCW) (2.08 which is (13.43, 13.86 and17.33 folds) higher than respective days exposure. This research suggests optimal elicitation could a good strategy large-scale these secondary metabolites industrial level.